响应面法优化半纤维素酶提取梨渣中可溶性膳食纤维工艺

Applying Response Surface Methodology to Optimize Extraction of Soluble Dietary Fiber from Pear Residue Using Hemicellulase

以砀山梨渣为原料,采用半纤维素酶水解法从梨渣中提取可溶性膳食纤维,并利用响应面法优化其提取条件。通过单因素试验考察液料比、酶添加量、酶解温度和酶解时间对可溶性膳食纤维提取率的影响。在单因素试验基础上,采用响应面法,利用Box-Behnken试验设计,对酶解工艺中各影响因素进行优化。结果表明,半纤维素酶水解法提取梨渣可溶性膳食纤维的最适提取工艺条件为:液料比13∶1(m L/g)、酶解温度58℃、酶解时间5 h、酶添加量35 U/g。在该条件下可溶性膳食纤维的提取率为15.21%,与理论值相差1.1%,表明实测值与理论值之间具有良好的拟合度。梨渣可作为一种优质膳食纤维的原料,半纤维素酶能有效用于梨渣中膳食纤维的提取。

Soluble dietary fiber(SDF) was extracted from pear residue by hemicellulase hydrolysis. The optimum extraction conditions for SDF were determined using response surface methodology(RSM). The effects of four crucial parameters, including liquid/material ratio, hemicellulase dosage, hydrolysis temperature and time, on the extraction rate of SDF were investigated by single factor experiments. A Box-Behnken design(BBD) consisting of 29 experimental points with five replications at the center point was used to investigate the interactive effects of the four independent variables at three levels ranging from 10:1 to 18:1, from 35 to 55 U/g, from 40 to 60 ℃, and from 4 to 6 h, respectively on extraction efficiency. The maximum extraction rate of SDF(15.21%), which agreed with the predicted value(15.38%), was obtained when the extraction experiment was carried out for 5 h at 58 ℃ with a hemicellulase dosage of 35 U/g at a liquid/material ratio of 13:1(m L/g). Thus, it is feasible to use hemicellulase to extract SDF from pear residue.