糖枣多糖的单糖组成分析

Analysis of the Monosaccharide Composition of Polysaccharides from “Tangzao” Jujube Fruits, a Local Cultivar in Hunan, China

研究糖枣多糖的单糖组成。以3 a生糖枣为试材,采用糖腈乙酸酯衍生化法,利用气相色谱仪对5个枣多糖组分进行单糖成分测定。结果表明,在糖枣多糖的单糖组成中,以木糖醇、葡萄糖、半乳糖所含比例较高,分别占单糖总含量的19.4%、18.5%、24.2%(组分DTC中葡萄糖比例高达36.4%);鼠李糖、核糖、阿拉伯糖和甘露糖,所占含量次之,分别为7.1%、6.5%、8.1%、7.9%;岩藻糖、木糖含量较低,分别只占总单糖质量的5.0%、3.4%(组分DTB1中岩藻糖只占0.80%,组分DTB2中木糖只占0.70%)。由此可知,糖枣多糖中单糖组成的差异性大。

This study aimed to analyze the monosaccharide composition of five polysaccharides(DTA, DTB1, DTB2, DTB3,and DTC) extracted from 'Tangzao' jujube fruits, a major local cultivar in Hunan, China. The polysaccharides were extracted from fruits of three-year-old jujube trees and analysis of the monosaccharide composition was performed by gas chromatography following derivatization with aldononitrile acetate. The results showed that the most predominant monosaccharides were glucose, xylitol, glucose, galactose, which accounted for 36.4%, 19.4%(as high as 36.4% in DTC), 18.5%, and 24.2% of the total monosaccharides, respectively, followed by rhamnose, ribose, arabinoseand mannose, which accounted for 7.1%, 6.5%, 8.1% and 7.9% of the total sugars, respectively, and then fucose and xylose with relative contents of only 5.0% and 3.4%, respectively(only 0.8% fucose was contained in DTB1 and only 0.7% xylose in DTB2). It can be seen that the monosaccharide composition of polysaccharides from 'Tangzao' jujubes is quite different.