花蛤中糖胺聚糖的分离与结构鉴定

Separation and Structure of Glycosaminoglycans from the Hard Clam Meretrix meretrix

目的:以花蛤为提取原料,用多酶水解法提取花蛤中糖胺聚糖,利用红外光谱法对提取物中的硫酸软骨素的结构进行分析。采用超高效液相色谱与电喷雾-质谱联用技术对糖胺聚糖类组分进行研究。方法:色谱柱ACQUITY UPLC BEH Amide(2.1 mm×100 mm,1.7μm);保护柱ACQUITY UPLC BEH Amide Van Guard(2.1 mm×5 mm,1.7μm);流动相乙腈-水(体积分数0.2%冰醋酸)的体积比为75∶25;流速0.1 m L/min;进样20μL。结果:超高效液相色谱-电喷雾-质谱法可快速定性分离糖胺聚糖组分。结论:本实验为糖胺聚糖化合物的结构鉴定提供了一种有效的分析方法。

Objective: To extract glycosaminoglycans(GAGs) from hard clams(Meretrix meretrix) by multi-enzymatic hydrolysis and to analyze the structure of chondroitin sulfate in the extract by infrared(IR) spectroscopy. Methods: The components of GAGs were examined by ultra high performance liquid chromatography-electrospray-mass spectrometry(UPLC-ESI-MS) using an ACQUITY UPLC BEH Amide chromatographic column(2.1 mm × 100 mm, 1.7 μm) and an ACQUITY UPLC BEH Amide Van Guard guard column(2.1 mm × 5 mm, 1.7 μm). The mobile phase was composed of a mixture of water and methanol(75:25, V/V) containing 0.2% glacial acetic acid at a flow rate of 0.1 m L/min. The injection volume was set as 20 μL. Results: Rapid separation of the components was achieved and their structures were determined by UPLC-ESI-MS. Conclusion: This paper provides an effective method to identify the structure of GAGs.