响应面法优化泡沫分离桑叶蛋白工艺

Optimization of Foam Separation of Mulberry Leaf Protein by Response Surface Methodology

利用响应面法对泡沫分离桑叶蛋白的工艺条件进行优化,在单因素试验的基础上,根据Box-Behnken试验设计原理,选取稀释倍数、离子强度、温度和pH值4个因素,依据回归分析法来确定各工艺条件的影响因素,以桑叶蛋白的回收率和富集比为响应值作响应面和等高线图,得出桑叶蛋白泡沫分离的最佳工艺条件为:稀释40倍、pH 5.5、离子强度0.18 mol/kg、温度25℃。在该工艺条件下,桑叶蛋白的实际回收率为92.50%,富集比为7.63。验证实验结果表明,泡沫分离法能够作为桑叶蛋白粗分离的一种新方法。

The foam fractionation of mulberry leaf protein was optimized by response surface methodology. The selection and optimization of Experiment factors affecting separation efficiency and their levels were carried out using combination of one-factor-at-a-time method, Box-Behnken factorial design and regression analysis. Based on the response surface and contour plots established with protein recovery and enrichment ratio as the response values, the optimum conditions for the separation of mulberry leaf protein were found to be dilution factor of 40, pH 5.5, ionic strength of 0.18 mol/kg, and separation temperature of 25 ℃. Under these conditions, the experimental values of protein recovery and enrichment ratio were 92.50% and 7.63, respectively. In conclusion, foam fractionation can provide a new method for protein separation from mulberry leaves.