几种氧载体对纳他霉素发酵的影响

Effects of Oxygen Vectors on Microbial Production of Natamycin

为有效改善纳他霉素发酵液中的氧传递速率,研究了几种氧载体对纳他霉素发酵的影响。结果表明,氧载体不能作为碳源被细胞利用,并能提高发酵体系溶氧量和传氧速率,有效促进单位细胞的纳他霉素产率的提高。在摇瓶发酵初期添加6%的大豆油、6%的橄榄油和9%的二甲基硅油,纳他霉素产量分别提高了56.8%、59.5%和72.5%。发酵初期添加6%的表面活性剂吐温-80可促进菌体生长,同时有效提高纳他霉素产量82.6%。

In order to effectively improve the oxygen transfer rate in the microbial production of natamycin, the effect of several oxygen vectors on Streptomyces gilvosporeus biomass and natamycin production was studied. The results showed that oxygen vectors and surfactants could not be used as carbon source although they were useful to improve dissolved oxygen and transfer rate, as well as the production of natamysin. The yield of natamycin could be improved by 56.8%, 59.5% and 72.5% after adding 6% soybean oil, 6% olive oil and 9% polydimethyls iloxane at the beginning of fermentation, respectively. With the addition of 6% Tween-80, the yield of natamycin was improved by 82.6% and growth-promoting effect on the strain was observed.