发状念珠藻胞外多糖的抑菌与抗炎作用

Antimicrobial and Antiinflammatory Activities of Extracellular Polysaccharides from Nostoc flagelliforme

采用BG-11培养液,悬浮培养发状念珠藻,提取发状念珠藻胞外多糖。通过测滤纸片周围抑菌圈直径大小,研究发状念珠藻胞外多糖抑菌作用;通过研究发状念珠藻胞外多糖对二甲苯致小鼠耳肿胀的影响及对角叉菜胶致小鼠足跖肿胀的影响,探究它的抗炎性能。结果表明,发状念珠藻多糖在一定的质量浓度范围内对研究的几种细菌及青霉和红曲霉都有一定的抑菌作用,而对根霉无抑制作用;其能够有效缓解二甲苯和角叉菜胶引起的小鼠耳部与足部炎症。发状念珠藻胞外多糖具有一定的抑菌抗炎性。

Extracellular polysaccharides from Nostoc flagelliforme in liquid suspension culture with BG-11 medium were obtained. By measuring the diameter of the inhibition zone using filter paper disc diffusion method, the antimicrobial activity of the exopolysaccharides against different bacteria and fungi were evaluated, and the anti-inflammatory activity was also tested by examining their effects on the mouse ear swelling induced by xylene and the swelling of hind paws in mice induced by carrageenin. The results showed that Nostoc flagelliforme extracellular polysaccharides could inhibit Penicillium and Monascus to a certain degree, but had no inhibitory activity on Rhizopus. Nostoc flagelliforme extracellular polysaccharides could effectively alleviate the inflammation of mouse ears and hind paws induced by xylene and carrageenin. Therefore, Nostoc flagelliforme extracellular polysaccharides have certain antimicrobial and antiinflammatory activities.