摘要
以大豆粉末磷脂为原料,采用无水乙醇萃取和氧化铝柱色谱相结合的技术,研究了高纯度磷脂酰胆碱的制备方法。乙醇萃取后磷脂酰胆碱的纯度为62.00%,得率为31.94%。主要考察了柱层析过程中的固定相、洗脱液浓度、上样量、料液比及洗脱液流速对分离效果的影响。结果表明:当固定相为100~200目三氧化二铝、洗脱液为90%乙醇、上样量为1 g/30 g、料液比为1∶12(g/m L)、洗脱液流速为3.0 m L/min时,磷脂酰胆碱的纯度可达到94.52%,回收率为83.71%。该研究结果为进一步探讨工业化制备磷脂酰胆碱的研究提供了技术支持和数据支撑。
Phosphatidylcholine(PC) was purified from soybean lecithin powder by ethanol extraction and aluminum column chromatography. After the extraction, the purity and yield of PC were 62.00% and 31.94%, respectively. The influence of important chromatographic parameters such as stationary phase, ethanol concentration, loading amount, solid-to-liquid ratio, and elution flow rate on separation efficiency was investigated. Highly purified PC with a purity of 94.52% and a yield of 83.71% was obtained under the optimized conditions: alumina powder(100–200 mesh) as the stationary phase, elution with 90% ethanol at a flow rate of 3.0 m L/min, loading amount of 1 g/30 g, and solid-to-liquid ratio of 1:12(g/m L). This study will hopefully provide technical support and data for industrial production of PC.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第10期6-10,共5页
Food Science
基金
国家自然科学基金青年科学基金项目(31301502)
河南省农业科学院优秀青年科技基金项目(2013YQ25)
关键词
磷脂酰胆碱
柱色谱
氧化铝
粉末磷脂
phosphatidylcholine
column chromatography
aluminum
soybean lecithin powder
