响应面法优化类球红细菌中番茄红素提取工艺

Optimization of Extraction Process for Lycopene from rhodobacter sphaeroides by Response Surface Methodology

利用响应面法研究有机溶剂提取类球红细菌中番茄红素的工艺。以每克菌体提取所得番茄红素的量作为响应值,在单因素试验基础上,选取皂化时间、料液比、超声时间及提取次数为自变量,采用响应面法研究各因素及其交互作用对类球红细菌番茄红素提取量的影响并建立了番茄红素提取的回归模型,确定了番茄红素提取工艺的最佳条件为以丙酮为提取溶剂、皂化时间28 min、料液比1∶86(g/m L)、超声时间11 min、提取次数2次。在此条件下,番茄红素提取量为9 326.48μg/g。本实验所得工艺方法切实可行,为类球红细菌工业化发酵生产番茄红素的进一步研究提供技术参考。

This study aimed to optimize the extraction of lycopene from rhodobacter sphaeroides by response surface methodology. With the extraction yield of lycopene per gram of cells as the response value, based on single factor experiments, response surface methodology was used to explore the effects of saponification time, liquid-to-material ratio, ultrasonic treatment time and extraction number as well as their interactions on the extraction rate of lycopene. As a result, a regression model was established and the optimal extraction parameters were determined as 28 min saponification, 11 min ultrasonic treatment and extraction performed twice using acetone as the extraction solvent with a material-to-liquid ratio of 1:86(g/m L). Under these extraction conditions, the extraction yield of lycopene was 9 326.48 μg/g. The extraction method developed in this study is feasible and will lay a foundation for further study of industrial production of lycopene by r. sphaeroides fermentation.