大孔树脂纯化山杏核壳总黄酮的工艺优化

Purification of Total Flavonoids from Shells of Wild Apricot with Macroreticular Resin

以山杏核壳为原料,采用大孔树脂纯化山杏核壳提取液中的总黄酮,利用单因素试验和正交试验优化D101大孔树脂纯化山杏核壳总黄酮的工艺条件。结果表明:在上样溶液质量浓度0.3 mg/m L、p H 2、流速2 m L/min的条件下进行纯化实验,大孔树脂对山杏核壳总黄酮的平均吸附率为75.20%。山杏核壳总黄酮的质量分数可达51.63%,表明D101大孔树脂对山杏核壳总黄酮有较好的纯化效果,且工艺重复性和稳定性良好。

The purification of total flavonoids extracted from the shells of wild apricot using D101 macroreticular resin was optimized using combination of single factor and orthogonal array experiments. The results showed that the optimum purification conditions were determined as follows: sample concentration, 0.3 mg/m L; p H 2; flow rate for elution, 2 m L/min. With these parameters, the purity of total flavonoids was 51.63%. Therefore, D101 macroreticular resin was effective in purifying total flavonoids from the shells of wild apricot with good stability and repetition.