解淀粉芽孢杆菌抗菌物质发酵培养基的优化

Optimization of Fermentation Medium for the Production of Antimicrobial Substances by Bacillus amyloliquefaciens

采用双向单因素试验法及正交试验法,以解淀粉芽孢杆菌K6为菌种,对发酵合成抗菌物质的改良兰迪培养基进行优化。结果表明:KCl对解淀粉芽孢杆菌K6抗菌活性物质的合成不利,Fe SO4和Cu SO4对抗菌活性物质的合成影响不大,而KH2PO4、Mg SO4和Mn SO4对抗菌活性物质的合成影响较显著。经优化获得适宜用于合成抗菌物质的兰迪培养基的配方为:L-谷氨酸钠5.0 g/L、葡萄糖15.0 g/L、KH2PO4 0.5 g/L、Mg SO4 0.2 g/L、Mn SO42.0 mg/L。优化兰迪培养基的组分较优化前的改良兰迪培养基显著减少,并更有利于抗菌活性物质的生物合成。

The modified Landy medium used for the production of antimicrobial substances by Bacillus amyloliquefaciens K6 was optimized by using a combination of bidirectional single factor method and orthogonal array design.The results indicated that KCl was unfavourable for the biosynthesis of antimicrobial substances,which was little affected by Fe SO4 and Cu SO4 but significantly affected by KH2PO4,Mg SO4 and Mn SO4.The optimum medium for the yield of antimicrobial substances consisted of 5.0 g/L L-monosodium glutamate,15.0 g/L glucose,0.5 g/L KH2PO4,0.2 g/L Mg SO4,and 2.0 mg/L Mn SO4.Compared with the initial Landy medium,the optimized Landy medium contained much fewer constituents but was more suitable for the biosynthesis of antimicrobial substances.