响应面法优化香蕉皮多糖的超声波辅助提取工艺

Optimizing Ultrasonic-assisted Extraction Process for Polysaccharides from Banana Peel by Response Surface Methodology

利用响应面分析法优化超声波辅助提取香蕉皮多糖工艺。苯酚—硫酸法测多糖含量,以单因素试验结果为依据,采用Box-Behnken试验设计优化香蕉皮多糖的提取工艺。结果表明:超声波辅助提取香蕉皮多糖的最优提取条件为液料比50∶1(mL/g)、超声波提取温度45℃,超声波提取时间24 min,超声波功率300 W,在此条件下多糖提取得率为7.15%。

The response surface methodology was used to optimize the ultrasonic assisted extraction process of polysaccharides from banana peel. The content of polysaccharide was determined by phenol-sulfuric acid method. On the basis of single factor experiment, the extraction process was optimized by Box-Behnken test. The results showed that the optimal extraction conditions of polysaccharide from banana peel were as follows: the ratio of liquid material was 50 ∶ 1(mL/g), the ultrasonic extraction was 45 ℃, the ultrasonic time was 24 min, the ultrasonic power was 300 W. Finally, the extraction rate of polysaccharide was 7.15%.