高效液相色谱法测定蜜饯中乙二胺四乙酸二钠的含量

Determination of Ethylenediamine Tetraacetic Acid Disodium Salt Content in Preserved Fruits by High Performance Liquid Chromatography

运用高效液相色谱法测定以蜜饯中乙二胺四乙酸二钠。样品用水溶解,加入氯化铁溶液,采用C18柱分离,以甲醇和0.075 mo L/L KH2PO4水溶液进行梯度洗脱,出峰时间定性,外标法定量。结果表明:该方法的乙二胺四乙酸二钠检出限为20 mg/kg,线性范围为250μg/m L,相关系数r=0.999 83,回收率为94.2%97.5%,相关标准偏差（RSD）0.47%。该方法前处理简便、灵敏度高、分离效果好、精确度高和精密度好,对蜜饯中乙二胺四乙酸二钠的含量测定更有针对性。

An analytical method for ethylenediamine tetraacetic acid disodium salt in preserved fruits by high performance liquid chromatography was developed.Using pure water,Fe Cl3 reagent, using C18 column separation, with methanol and 0.075 mol/L KH_2PO_4 solution gradient elution,high performance liquid chromatography was used to qualititative and quantitative analysis.Results show that the method of ethylenediamine tetraacetic acid disodium detection limit of 20 mg/kg, the linear range is 2 ~ 50 μg/m L, correlation coefficient r = 0.999 83, the recovery rate of 94.2% ~ 97.5%, relative standard deviation(RSD) is 0.47%. The method is accurate,repeatable and suitable for the determination of ethylenediamine tetraacetic acid disodium salt in preserved fruits.