不同方法提取马齿苋多糖的抗氧活性比较

The Comparison of Antioxidant Activity of Polysaccharides Extracted from Purslane by Different Ways

用不同方法提取马齿苋多糖,并测定多糖含量及其粗多糖中蛋白质含量。采用体外试验研究马齿苋多糖对Fe^(3+)、Ce^(4+)的还原能力以及对超氧阴离子(O_2^-·)和羟自由基(·OH)的清除作用。结果表明,不同方法提取多糖的得率为:热水浸提>木瓜蛋白酶>纤维素酶>果胶酶>超声波辅助提取。其粗多糖中蛋白质含量是:果胶酶>纤维素酶>超声波辅助提取>热水提取>木瓜蛋白酶。在试验浓度范围,不同方法提取的马齿苋粗多糖对Fe^(3+)和Ce^(4+)的还原能力,热水提取最强,其次是果胶酶;用果胶酶提取的粗多糖对超氧阴离子自由基清除能力最强,其次是纤维素酶;对羟基清除能力,添加量为2.0 m L时,纤维素酶最好,添加量为1.2 m L时,果胶酶最好。从这4个方面综合来评价,果胶酶提取的马齿苋粗多糖抗氧化性最好。

Purslane polysaccharide extracted by different methods,and determination of the content of polysaccharide and protein content. Using in vitro experimental study of purslane polysaccharide on Fe^(3+), Ce^(4+)reduction ability and the superoxide anion(O_2^-·) and hydroxyl radical(·OH) scavenging effect. The results showed that, for the rate of polysaccharide extracted with different methods: hot water extraction > papain >cellulase >pectinase >ultrasonic-assisted extraction. The protein content of the crude polysaccharide: pectinase >cellulase > ultrasonic extraction > hot water extraction > papain. In the experimental concentration range, the reduction capability of purslane polysaccharide on Fe^(3+) and Ce^(4+): hot water extraction was strongest, followed by pectinase. The crude polysaccharide scavenging superoxide anion free radical by pectinase extraction had the strongest ability, followed by cellulose. Adding amount was 2.0 m L, cellulase on hydroxyl scavenging ability was the best, adding amount was 1.2 m L, the best of pectinase. From these four aspects to evaluate, pectinase extraction of purslane crude polysaccharide antioxidation was the best.