摘要
阪崎克罗诺杆菌是食源性条件致病菌,它能够在食品生产环境以及设备表面形成生物膜,从而成为食品的潜在污染源。本研究探讨了盐酸小檗碱对阪崎克罗诺杆菌生物膜的抑制作用,旨在从中药来源中寻找可以控制阪崎克罗诺杆菌生物膜的抗菌物质。采用XTT法评价了盐酸小檗碱对阪崎克罗诺杆菌粘附能力及其生物膜形成的影响。盐酸小檗碱对阪崎克罗诺杆菌的抑制效果表现出了剂量依赖性,MIC_(90)值为512μg/m L。浓度为1 024μg/m L的盐酸小檗碱能够通过降低阪崎克罗诺杆菌的粘附能力从而显著抑制生物膜的形成,并且可以部分清除阪崎克罗诺杆菌成熟的生物膜。因此,盐酸小檗碱对于预防控制阪崎克罗诺杆菌生物膜的形成以及由生物膜导致的临床感染具有潜在的应用价值。
Cronobacter sakazakii is an emerging pathogen which can form biofilms on the surfaces of equipment and processing environments. The biofilms of C. sakazakii are a potential source of contamination in food. In this study,the effect of berberine to inhibit C. sakazakii biofilm formation was investigated,which can help find antimicrobial substances from Chinese medical herbs to control its biofilms. The effect of berberine hydrochloride on the initial adhesion of C. sakazakii and its biofilm formation was evaluated by XTT reduction assay. The results revealed that the inhibition effect of berberine hydrochloride on C. sakazakii biofilms showed a dose dependent relationship and the MIC_(90) of berberine hydrochloride against C. sakazakii was 512 μg/m L. Berberine hydrochloride at a concentration of 1 024 μg/m L could inhibit the early adherence of C. sakazakii,which suppressed the development of new biofilms. At this concentration,berberine hydrochloride could also partially destroy the mature biofilms. Therefore,berberine hydrochloride showed a great potential in prevention and control of biofilm formation of C. sakazakii and the biofilm-related infections caused by this pathogen.
出处
《食品研究与开发》
CAS
北大核心
2016年第17期134-138,共5页
Food Research and Development
基金
国家高技术研究发展计划(863)课题(2012AA101703)
