不同品系猴头菇子实体中总酚含量测定

Determination of Total Polyphenol Content from Different Hericium erinaceus Strains

该文以猴头菇子实体为原料,采用超声波辅助提取法优化猴头菇总酚提取工艺条件。采用Folin-Ciocalteu比色法测定总酚的含量,没食子酸为标准品,以猴头菇子实体中总酚的含量为评价指标,分别对提取溶剂、提取时间和料液比进行优化,建立了猴头菇子实体中总酚的含量测定方法,得到最佳料液比1∶40(g/m L),纯水提取,提取时间60 min,总酚含量在0~14μg时与吸光度呈良好的线性关系,其回归方程Y=0.037 3X+0.003 7,r≥0.999,平均加样回收率为101.41%,RSD为1.20%。同时,将该方法在最适条件下测定了10个不同品系猴头菇子实体中总酚的含量,不同品系猴头菇子实体中总酚的含量在2.20 mg/g^4.77 mg/g之间。结果表明,该方法简单、快速、重复性好,可用于猴头菇子实体的总酚含量测定方法。

The total polyphenol content from Hericium erinaceus was abstracted by ultrasonic-assisted extraction. The total polyphenol content was analyzed by Folin-Ciocalteu(FC)colorimetry method,and gallic acid was used as reference substance. Effects of extraction solvent,extraction time and solid to liquid ratio on total polyphenols content from Hericium erinaceus were investigated. It showed that the optimized extraction conditions were water extract,the extraction time was 60 min and the solid-liquid ratio is 1 ∶ 40(g/m L). The calibration curves were linear over gallic acid content ranging from 0 ~14 μg,the typical standard curves were Y =0.037 3X+0.003 7 with the line regression correlation coefficients greater than 0.999. The average recovery was101.41 % with RSD of 1.20 %. This method was also successfully applied to the determination of total polyphenols content from ten different Hericium erinaceus strains. The total polyphenol content from different Hericium erinaceus strains between 2.20 mg/g^4.77 mg/g.This method established is simple,rapid and repeatable,which is suitable for analysis of total polyphenols in Hericium erinaceus.