摘要
对影响天麻多糖提取率的酶用量、提取时间、提取温度及pH值进行单因素试验,并使用正交法得到酶法提取天麻多糖最佳工艺。结果表明,提取天麻多糖最佳工艺为酶用量8 mg/g,提取时间110 min,提取温度为40℃,pH值为4.5,在最优提取工艺条件下,天麻多糖提取量为46.63 mg/g。
Influencing factors which affected the extraction yield of polysaccharide in Gastonia elate was studied,including enzyme amount,temperature,time,and pH,and the extraction conditions of polysaccharide in Gastonia elate were optimized by orthogonal experiments. Result as follows:the enzyme amount was 8 mg/g,the enzymatic hydrolysis time was 110 min,the enzymatic extraction temperature was 40 ℃,the pH was 4.5,and the polysaccharide in Gastonia elate was 46.63 mg/g under the optimum conditions.
出处
《食品研究与开发》
CAS
北大核心
2017年第15期50-53,共4页
Food Research and Development
基金
铜仁市科技局计划项目(铜市科研[2014]40-6)
铜仁学院食品科学与工程校级重点支持学科建设项目(0002)
贵州省教育厅大学生创新创业训练中心项目(2015SJDCZX001)
贵州省教育厅资助(黔学位办[2013]18号)
关键词
天麻
酶解法
正交试验
多糖
Gastonia elate
enzymatic method
orthogonal experiments
polysaccharide
