草果总黄酮的提取及DPPH自由基清除活性研究

Extraction Method and DPPH Radical Scavenging Activity of Flavonoids from Amomum tsaoko

采用超声波辅助法提取草果总黄酮,通过单因素试验和正交试验优化草果黄酮的最佳提取条件,同时以VC为对照,评价草果黄酮清除1,1-二苯基-2-苦基肼(1,1-Diphenyl-2-picryl-hydrazyl,DPPH)自由基的能力。结果表明草果黄酮的最佳提取条件为:乙醇体积分数60%、料液比1∶50(g/mL)、提取温度40℃、超声功率为160 W,提取时间60 min,此时草果黄酮提取量是24.2 mg/g。草果黄酮有较强的DPPH自由基清除力,且草果黄酮抗氧化性高于VC,其IC50分别为:IC50草果黄酮12.89 mg/L,IC50VC为6.94 mg/L,草果黄酮的DPPH自由基清除率为80.5%。

Ultrasonic assisted method was used to extract total flavonoids from Amomum tsaoko. Through the single factor experiment and orthogonal experiment,the best conditions to extract flavonoids were found. At the same time,using the assay system of 1,1-Diphenyl-2-picryl-hydrazyl(DPPH),the antioxidant activities of the Amomum taroko flavonoids were studied and compared with those of VC. Results showed that optimum extraction conditions for total flavonoids from Amomum tsaoko was that volume fraction of 60 % ethanol,ratio of material to liquid was 1 ∶ 50(g/mL),the temperature was 40 ℃,the ultrasonic power was 160 W and the extraction time was 60 min. On this conditions,the total flavonoids extraction quantity was 24.2 mg/g. The Amomum tsaoko flavonoids and VCboth have strong DPPH radical scavenging ability,and the scavenging activity was VC>Amomum tsaoko flavonoids,and the IC50 amomum flavonoids was 12.89 mg/L,the IC50 VCwas 6.94 mg/L,the DPPH radical scavenging rate of was 80.5 %.