睡莲花露主要成分及抗氧化作用

The Main Metabolites and Antioxidation Effect of Waterlily Stigmatic Exudate

以‘蓝鸟’睡莲(Nymphaea ‘Blue Bird’)花露为材料,采用液相色谱-质谱(liquid chromatograph-mass spectrometer,LC-MS)法,分析睡莲花露的主要成分,同时采用1,1-二苯基-2-三硝基苯肼(1,1-dipheny1-2-picrylhydrazyl,DPPH)法、铁离子还原/抗氧化能力(ferric reducing ability of plasma,FRAP)法、总抗氧化能力(total peroxyl radical-trapping antioxidant parameter,TRAP)法测定睡莲花露的抗氧化能力。代谢成分分析结果表明,睡莲花露的主要成份有葡萄糖酸、马来酸甲麦角新碱,单半乳糖甘油二酯,硫代异鼠李糖甘油二酯、1-十六酰-SN-丙三醇-磷酸胆碱、东莨菪内酯、威严仙皂苷C、葡萄糖醛酸、槲皮素、肌醇半乳糖苷和香豆素等多种物质。抗氧化能力测试表明,DPPH法中350μL睡莲花露的自由基清除率为(85.19±1.55)%;FRAP法中测定的睡莲花露抗氧化能力相当于6.35 mmol/L的FeSO_4的抗氧化能力;TRAP法中花露加入量与TRAP值呈线性相关,5μL的睡莲花露加入量其抗氧化能力相当于1.425 2 mmol/L的抗坏血酸钠。

The main components of the stigmatic exudate of Nymphaea’Blue Bird’were analyzed by the LC-MS method with MS-Dial software,and three methods of DPPH(1,1-dipheny1-2-picryl-hydrazyl),FRAP(ferric reducing ability of plasma)and TRAP(total peroxyl radical-trapping antioxidant parameter)were used to determine the antioxidant capacity of waterlily stigmatic exudate.Results showed that the waterlily stigmatic exudate was composed of D-gluconic acid,methergine,monoglycosyldiglycerides,sulphoquinovosyldiglyceride,lysoPC 18:2,7-hydroxy-6-methoxychromen-2-one,cauloside C,glucuronic acid,quercetin,coumarin and other metabolites.The results of the antioxidant capacity showed that the free radical scavenging activity of 350μL waterlily stigmatic exudate was(85.19±1.55)%by the DPPH method.The antioxidant capacity of waterlily stigmatic exudate was equivalent to the antioxidant capacity of 6.35 mmol/L FeSO4 by FRAP method.In the TRAP method,the amount of stigmatic exudate was linearly correlated with the TRAP value,and the antioxidant capacity of 5μL stigmatic exudate was equivalent to 1.425 2 mmol/L ascorbic acid.