禽传染性支气管炎病毒结构蛋白在Vero细胞中的表达效果分析

Expression of Structural Proteins of Infectious Bronchitis Virus in Vero Cells

将IBV的S1基因、N基因分别构建到pCI-neo等真核表达载体中,并转染了Vero细胞.通过间接免疫荧光实验及细胞免疫化学实验证实了IBV的S1蛋白、N蛋白均可在Vero细胞中进行表达,24h^72h间均检测到蛋白的表达.结果显示S1蛋白在Vero细胞中的表达量低于N蛋白,pCI-neo载体的表达效果优于pcDNA3载体.此外,还探讨了S1基因与具有免疫调节作用的鸡白细胞介素-2基因在Vero细胞中串联方式共表达,成功检测到两种蛋白,为探讨DNA疫苗使用方法提供了一条潜在的途径.

S1gene and N gene of IBV are ligated with pCI-neo vectors respectively and transfected into Vero cells.IFA and ICC assays confirm that both S1and N protein are expressed 24hs to 72hs after transfection.How genes and vectors effect expression of proteins is briefly evaluated.It is observed that N protein shows a higher expression level compared with S1protein and pCI-neo vector offering higher expression level compared with pcDNA3vector.In addition,S1gene is successfully co-expressed in Vero cells with an immuno-modulator chicken Interluekins-2,which provides a potential administration method of DNA vaccine.