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白蚁栖息地一株纤维素降解菌的筛选和酶活性分析 被引量：2

Isolation and Characterization of a Strain with Cellulose-degradation Activity from a Termite-inhabiting Soil
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摘要 从白蚁栖息地土壤中筛选分离得到一株具有纤维素降解活性的细菌ZJSRU-11,对其进行分子生物学鉴定,并研究其粗酶液的酶学性质.该菌株初步鉴定为无色杆菌(Achromobacter sp.),具有完整的纤维素酶系统,包括羧甲基纤维素钠酶活力、滤纸酶活力和β-糖苷酶活力.ZJSRU-11菌株羧甲基纤维素钠酶活力较高,在45℃、pH 5.8的反应条件下,反应35min时活力达到19.6U/mL. A microorganism hydrolyzing carboxymethyl cellulose is isolated from the termite-inhabiting soil and identified according to phylogenetic analysis.The characterization of crude CMCase is examined.The strain is identified as the Achromobacter sp and has a complete cellulase system including FPase,CMCase andβ-glucosidase.The strain ZJSRU-11 has high CMCase activity and exhibits its optimal activity of 19.6U/mL at 45℃and pH 5.8after 35min.
作者 陈康丽 张建芬 陈虹
机构地区 浙江树人大学生物与环境工程学院
出处 《浙江树人大学学报（自然科学版）》 2013年第4期20-23,27,共5页 Journal of Zhejiang Shuren University(Acta Scientiarum Naturalium)
基金 2012年度浙江省大学生科技创新项目(2012R420023)
关键词 纤维素降解菌 白蚁 羧甲基纤维素钠酶 无色杆菌 Cellulose-degrading bacteria termite CMCase Achromobacter sp.
分类号 Q93 [生物学—微生物学]
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参考文献5

  • 1何刚强,堵国成,刘立明,霍光华.从白蚁中分离筛选纤维素分解菌及其产酶性质[J].食品与生物技术学报,2009,28(3):352-355. 被引量：20
  • 2陈虹,梅建凤,闵航.白蚁肠道微生物[J].微生物学杂志,2005,25(2):75-79. 被引量：32
  • 3Kun Zhao,Li-Zhong Guo,Wei-Dong Lu.Extracellular Production of Novel Halotolerant, Thermostable, and Alkali-Stable Carboxymethyl Cellulase by Marine Bacterium Marinimicrobium sp. LS-A18[J].Applied Biochemistry and Biotechnology.2012(3)
  • 4Muhammad Rizwan Javed,Muhammad Hamid Rashid,Habibullah Nadeem,Muhammad Riaz,Raheela Perveen.Catalytic and Thermodynamic Characterization of Endoglucanase (CMCase) from Aspergillus oryzae cmc-1[J].Applied Biochemistry and Biotechnology.2009(3)
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二级参考文献36

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浙江树人大学学报（自然科学版）
2013年 第4期

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