玉米低聚肽硒螯合物的体外抗氧化作用

Antioxidant effects of selenium-chelating corn oligopeptide in vitro

以玉米低聚肽和亚硒酸钠为原料经螯合工艺制得玉米低聚肽硒螯合物,对其水分、粗蛋白、酸溶蛋白、分子量分布情况进行了考察,然后从DPPH自由基、OH自由基清除能力和还原能力3方面对螯合原料和螯合物进行抗氧化功能对比与评价。结果表明,螯合得率为53.02%±0.17%,水分含量为7.46%±0.07%,酸溶蛋白占粗蛋白的比例为77.38%,分子量分布在1000u以下的比例超过85%。螯合原料中亚硒酸钠仅对OH自由基有较强清除能力;玉米低聚肽对DPPH自由基、OH自由基均有一定清除能力,且具有一定还原性;螯合物清除DPPH自由基、OH自由基能力及还原能力均比玉米低聚肽强,且具有剂量依赖。

Selenium-chelating corn oligopeptide were prepared by corn oligopeptide and sodium selenite. The properties of moisture, acid soluble protein, total nitrogen and molecular weight distribution were analyzed. Taking scavenging effects on DPPH, OH free radicals and reducing power as indicators, the antioxidant function of raw materials and chelates were evaluated and compared in vitro. The results showed that the yield was 53.02%±0.17%, the moisture content of the product was 7.46%±0.07%, the acid soluble protein accounted for 77.38% of the crude protein, and the molecular weight below 1000 u was more than 85%. In the raw materials, sodium selenite only had strong OH radicals scavenging ability.Corn oligopeptide had some scavenging ability on DPPH and OH radicals, and had some reducibility.Compared with corn oligopeptide, the selenium-chelating corn oligopeptide had better ability on scavenging DPPH and OH radicals, and reducing power, which were dose-dependent.