HPLC测定猕猴桃不同部位中的7种多酚类化合物

Determination of polyphenols in kiwifruit by HPLC

目的:建立一种能同时测定猕猴桃皮、茎、叶、果肉中7种多酚类化合物的分析方法。方法:多酚类化合物经60%甲醇萃取后,进行HPLC定性定量分析。HPLC检测条件为:流动相A为甲醇,B为0.5%乙酸,检测波长280 nm,采用梯度洗脱程序。结果表明:各多酚类化合物线性相关系数R2≥0.9992,均具有良好线性,加标回收率在95.25%~101.22%,相对标准偏差(RSD)小于2%,方法的检出限在0.002~0.007 mg/g。猕猴桃中含有丰富的多酚类化合物,其中猕猴桃皮、茎、叶、果肉中含量最高的多酚类化合物分别是原儿茶酸、七叶亭、七叶亭、表儿茶素。结论:该方法分离效果好,分析准确度高,重现性好,因此可进一步应用于猕猴桃其他酚类物质的分析;猕猴桃皮中酚类化合物最丰富,可进一步加强利用。

Objective:To establish a method for simultaneous determination of 7 polyphenols in different parts of kiwifruit.Method:The detection conditions for kiwi polyphenol by high performance liquid chromatograph(HPLC)were as follows:The mobile phase A was methanol,B was 0.5%acetic acid,the detection wavelength was 280 nm,and the gradient elution process was adopted.Results:The recoveries of standard addition ranged from 95.25%to 101.22%,with relative standard deviation(RSD)was less than 2%.The linear relationship was good with determination coefficients R2≥0.9992 in the 7 polyphenols.The detection limit of the method was 0.002~0.007 mg/g.The most abundant polyphenols in kiwifruit peel,stem,leaf and pulp were protocatechuic acid,esculetin,esculetin,epicatechin.Conclusion:This method can be further developed and applied to the detection of other polyphenols in kiwifruit.The polyphenols in kiwifruit peel are the most abundant and can be further utilized.