摘要
从患病的克氏原螯虾体内分离到一株致病菌L2M-A,经生理生化鉴定和16S rDNA序列分析,证实菌株L2M-A为豚鼠气单胞菌(Aeromonas caviae)(GenBank登录号:KF446251),其16S rDNA序列与基因库中气单胞菌属菌株的16S rDNA序列有99%—100%的同源性,而且与豚鼠气单胞菌JXZ-3株(GenBank登录号:JF496552)的亲缘关系最近。此外,菌株L2M-A在pH5—9内均能够生长良好,最适生长温度为30℃,最适生长转速为200 r/min,但浓度≥6.25μg/mL的双氟沙星对其生长具有显著的抑制作用,可作为防治用药的依据。
A pathogenic strain L2M-A was isolated from diseased Procambarus clarkii, and was identified as an Aero-monas caviae strain (GenBank accession No. KF446251) with biochemical and molecular approaches. The 16S rDNA sequence of this strain showed a homology of 99%to 100%with those of Aeromonas strains, and was closest related to that of A. caviae strain JXZ-3 (GenBank accession No:JF496552). We also found that the optimal growth conditions of strain L2M-A were pH5-9, temperature at 30℃, and spinning speed at 200 r/min. The growth of L2M-A could be sig-nificantly inhibited by difloxacin at the concentration of ≥6.25 μg/mL. This finding could provide scientific guidance for the disease control.
出处
《水生生物学报》
CAS
CSCD
北大核心
2014年第6期1047-1053,共7页
Acta Hydrobiologica Sinica
基金
江苏省科技支撑计划项目(No.BE2013366)
山西省科技攻关项目(No.20120311025-4)
上海高校实验技术队伍建设计划(个人)项目
上海海洋大学水产类人才创新创业实训基地(泗洪)项目
国家自然科学基金青年基金项目资助
关键词
豚鼠气单胞菌
鉴定
生物学特性
克氏原螯虾
Aeromonas caviae
Identification
Biological characteristics
Procambarus clarkii
