产纤维素酶菌株产酶条件的研究

Study on the Conditions of Producing Cellulose by Trichoderma uirde

主要研究了绿色木霉固态发酵培养基的配比、接种量、氮源、培养时间和培养温度等条件 ,通过测定其所产纤维素酶的CMC和FPA酶活 ,找出该菌株最佳的产酶条件 ,即 :秸秆粉∶麸皮为 2∶1,固液比为 1∶3,添加 (NH4) 2 SO4为氮源 ,添加量为 2 % ,接种量 6 % ,30℃培养84h左右为宜。CMC、FPA酶活分别达到 372IU/g和 6 9IU /g。

In this text the factors including media, water contents,nitrogen sources, cultural temperature and culture time were studied for producing cellulose by the mutant stain of Trichoderma uirde. The results shows that the optium condition of solid fermentation is that straw pauden 10 g, wheat bran 5 g, water 30 ml, (NH 4) 2SO 4 0.2 g, and at 30℃ for 84 h. Under this condition, the enzyme activities rate towards CMC、FPA reached 372 IU/g and 69 IU/g.