NMR、FTIR及电位滴定法测定壳聚糖脱乙酰度

Degree of Deacetylation of Chitosan Determined by Solid Method and Patentiometric Titration

脱乙酰度是影响甲壳素、壳聚糖及其衍生物物化特性和生物活性功能的重要参数。介绍了三种壳聚糖脱乙酰度的测定方法。以13C交叉极化魔角旋转固体核磁法测定的结果作为参照,对红外光谱法和线性电位滴定法的测定结果进行了分析比较,并分析了不同测定方法的误差来源。

Degree of deacetylation (DD) is a key index to chitosan and its derivatives for their physiochemical and bioactive properties. In this study, three methods of measuring DD suck as patentiometric titration, FTIR and 13C CP/MAS NMR were introduced. In comparison with the DD determined by 13C CP/MAS NMR, the results obtained by the other two methods have been analyzed and the sources of the deviations were suggested.