哈茨木霉NF9菌株对水稻的诱导抗病性

Resistance Induced by Trichoderma harzianum NF9 Against Magnaporthe grisea and Xathomonas oryzae pv. oryzae in Rice

在温室条件下用哈茨木霉NF9菌株进行种子处理,诱导水稻感病品种原丰早幼苗对稻瘟病和白叶枯病的抗病性,其抗性水平为中抗,对稻瘟病菌不同菌株(SY1、H16)以及对白叶枯病菌不同菌株(CR1、CR7)的抗性表现无显著差异。经木霉种子处理的水稻幼苗接种稻瘟病菌或白叶枯病菌后,过氧化物酶及苯丙氨酸转氨酶活性增强,在某一时段显著高于未经木霉种子处理的对照。诱导抗性可能是哈茨木霉NF9菌株的生防机制之一。

In greenhouse experiments, susceptible rice cultivar Yuanfengzao seedlings pretreated (in seedcoating) with Trichoderma harzianum NF9 exhibited moderate resistance against blast and bacterial leaf blight diseases after challenge inoculation with corresponding pathogens in comparison with susceptible response in seedlings untreated with the NF9 strain. Enzyme activities of PAL and POD in rice seedlings pretreated with the Trichoderma strain were increased after challenge inoculation with pathogens. At 120 h after inoculation with both Magnaporthe grisea strain SY1 and H16, POD activities in NF9 treated rice seedlings were significantly higher than that in untreated ones, whereas no significant diffrence in PAL activity were found between two ones. At 16 h and 96 h after inoculation with Xathomonas oryzae pv. oryzae CR1, and at 120 h after inoculation with X. oryzae pv. oryzae CR7 PAL activity in NF9 treated rice seedlings was significantly higher than that in untreated ones, whereas no significant diffrence in POD activity were found between two ones.