miR-124a、let-7表达与胃癌临床病理特征和化疗疗效的相关性

Correlation of miR-124a and let-7 Expression with the Clinicopathological Features and Chemotherapeutic Efficacy of Patients with Gastric Cancer

目的:探讨外周血miR-124a和let-7的表达与胃癌临床病理特征和化疗疗效的相关性。方法:选取2013年1月～2017年3月在我院接受化疗的晚期胃癌患者为研究对象,所有患者入院后均择期行XELOX方案(奥沙利铂+卡培他滨)化疗。化疗前后,采用实时荧光定量PCR检测患者外周血miR-124a和let-7表达,分析其与患者临床病理特征的关系。根据化疗疗效将患者分为疾病控制组和疾病进展组,分析外周血miR-124a和let-7表达与化疗疗效的关系。结果:外周血miR-124a和let-7表达与肿瘤大小、分化程度、TNM分期和淋巴转移有关(P<0.05)。肿瘤≥3 cm、中低分化、TNM分期Ⅳ期和有淋巴转移的患者外周血miR-124a和let-7表达量更低。与化疗前比较,化疗后患者外周血miR-124a和let-7相对表达量显著增加,差异均有统计学意义(P<0.05)。95例患者,CR 0例(0.0%),PR 12例(12.6%),SD 20例(21.1%),PD 63例(66.3%),ORR为12.6%,DCR为33.7%。疾病控制组化疗后外周血miR-124a和let-7相对表达量明显高于疾病进展组,差异均有统计学意义(P<0.05)。Spearman秩相关分析显示外周血miR-124a和let-7相对表达量与化疗疗效呈正相关(rs=0.613、0.574,均P<0.05)。结论:晚期胃癌患者外周血miR-124a和let-7的表达与肿瘤大小、分化程度、TNM分期、淋巴转移和化疗疗效相关。

Objective: To investigate correlation of miR-124 a and let-7 expression with the clinicopathological features and chemotherapeutic efficacy of patients with gastric cancer. Methods: A total of 95 patients with gastric cancer who were treated in our hospital from January 2013 to March 2017 were selected as the research subjects. All of the patients were treated with XELOX(oxaliplatin+capecitabine) chemotherapy after admission. The expression of miR-124 a and let-7 in peripheral blood were detected by real-time fluorescence quantitative PCR before and after chemotherapy. The relationships between miR-124 a and let-7 and clinicopathological features were analyzed. All patients were assigned into the disease control group and the disease progression group according to the chemotherapy efficacy. The relationship between miR-124 a and let-7 and the chemotherapy efficiency were analyzed. Results: The expression of miR-124 a and let-7 in peripheral blood were correlated with the tumor size, differentiation, TNM stage and lymph node metastasis(P<0.05). The expression of miR-124 a and let-7 was lower in patients with tumor size(≥3 cm), moderately and poorly differentiated, high TNM stage(Ⅳ stage), lymph node metastasis. After chemotherapy, 0 cases complete remission, 12(12.6%) cases partial remission(PR), 20(21.1%) cases stable disease(SD), 63(66.3%) cases progressive disease(PD), the objective response rate(ORR) was12.6%, disease control rate(DCR) was 33.7%. The expression of miR-124 a and let-7 in peripheral blood in the disease control group was high than that in the disease progression group(P<0.05). Spearman rank correlation analysis showed that the relative expression of miR-124 a and let-7 in peripheral blood were positively correlated with chemotherapy efficacy(rs=0.613, 0.574, P<0.05). Conclusion:The expression of miR-124 a and let-7 in peripheral blood of patients with advanced gastric cancer were related to the tumor size, differentiation, TNM stage, lymph node metastasis and chemotherapy efficacy.