PARP-1抑制剂3-AB对肝癌细胞增殖及凋亡的影响

The Effect of PARP-1 Inhibitor 3-AB on Hepatocellular Carcinoma Cell Lines

目的:探讨PARP-1抑制剂3-AB对肝癌细胞系MHCC97-H和SMMC7721及正常肝细胞系L02的增殖与凋亡的影响。方法:细胞增殖试验观察不同浓度3-AB对三种不同细胞系细胞的增殖作用。Annexin V荧光探针标记,流式细胞学检查观察不同浓度3-AB对不同细胞系细胞凋亡的影响。结果:当3-AB浓度分别为5 mM、10 mM与20 mM时,与对照组(0 mM)相比,在培养第6天时开始出现增殖明显减慢,出现统计学差异(p<0.05),第九天差异明显(p<0.05)。随着浓度增加,其对肿瘤细胞系MHCC97-H和SMMC7721细胞增殖的抑制程度增加,细胞数均逐渐减少;而同样浓度梯度3-AB对人类肝细胞系L02生长则无明显的抑制作用。进一步实验发现,当3-AB浓度为5mM、10 mM与20 mM时,均可诱导肝癌细胞株MHCC97-H和SMMC7721凋亡,与对照组(0 mM)比较均有统计学差异(p<0.05),且细胞凋亡率与3-AB的药物浓度相关:浓度越高,凋亡越明显。而同等浓度3-AB对肝脏细胞系L02无明显的促进凋亡作用。结论:3-AB可以抑制肝癌肿瘤细胞的增殖,促进肿瘤细胞的凋亡,对正常肝脏细胞无明显毒害作用,具有治疗肝癌的的潜在应用价值。

Objective: To study the effect of parp-1 inhibitor 3-AB on the proliferation and apoptosis of the hepatocellular carcinoma cell lines of MHCC97-H, SMMC7721 and hepatocellular cell line L02. Methods: Cell proliferation assay was conducted to observe the effects of 3-AB on L02, MHCC97-H and SMMC7721 cell growth. FACS was used to evaluate the apoptosis ratio of different cell lines of human liver cancer cell lines and hepatocellular cell line L02 with different 3-AB dosage. Results: 3-AB can significantly inhibit the growth rate of MHCC97-H and SMMC7721 cell lines. As the concentration increased, the suppressive effects of 3-AB were increasing. The growth inhibition was significant on the 6 th and 9 th day of culture. Compared with the control group(0 mM), the growth rate of MHCC97-H and SMMC7721 cell lines with different concentrations of 3-AB(5 mM, 10 mM and 20 mM)(p<0.05) were lower on the 6 th day and from then on;3-AB did not affect the growth rate of L02 cell line in the same concentration(p>0.05). Compared with the control group(0 mM), the apoptosis ratio of MHCC97-H and SMMC7721 cell lines were higher than the other 3 groups with different concentration of 3-AB(5 mM, 10 mM and 20 mM)(p<0.05). Yet it has no effect on the apoptosis of L02 cell line in the same concentration(p>0.05). Conclusion: 3-AB can inhibit the growth rate of MHCC97-H and SMMC7721 cell lines and induce the cells apoptosis. And the apoptosis ratio was variable with the different 3-AB concentrations. But different concentrations had no effect on the growth or apoptosis of L02 cell line. 3-AB can be a potential good choice for liver cancer therapy and maybe improve the patients life quality.