L型多聚赖氨酸和D型多聚赖氨酸对神经细胞分化的影响

Influence of L-PL and D-PL on Nerve Cell Differentiation

目的:比较L型多聚赖氨酸(L-PL)和D型多聚赖氨酸(D-PL)浸泡的玻片培养小鼠大脑皮层神经元细胞对其分化的影响。方法:取昆明白小鼠的大脑皮层细胞,分别用L-PL和D-PL进行培养,统计和比较细胞的分化比率、突起数量以及突起生长长度。结果:L-PL和D-PL培养的神经细胞第1、2天的分化率比较差异具有统计学意义(P<0.05),而突起数和突起长度比较差异无统计学意义(P>0.05)。当L-PL、D-PL的浓度分别为20μL/m L、5μL/mL时,其对1、2天细胞的分化产生显著影响,并且D型多聚赖氨酸培养的细胞分化率高于L型。500、100、20μg/m L的L-PL中,100μg/ml L-PL培养细胞的分化率最高,而125、25、5μg/m L的D型多聚赖氨酸培养细胞分化率比较差异虽然无统计学意义,但25μg/ml D-PL培养神经细胞的分化率总体趋势高于其他浓度。D-PL所形成的粒径大小与L-PL不同,且D-PL的总体趋势稍大于L-PL。结论:L型多聚赖氨酸和D型多聚赖氨酸会在神经细胞生长早期对分化率产生影响,但不影响突起数和突起长度。

Objective:To compare the effect of L-PL and D-PL soaked glass on the differentiation of nerve cells.Methods:The cerebral cortex cells of KM mice were cultured with D-PL and L-PL,respectively,the cell differentiation rate,branch number and branch length were counted.Results:The results showed that the differences of the differentiation rate but not the number and length of protuberances of nerve cells was significantly significant at day 1 and 2 between L-PL and D-PL(P<0.05).When the concentrations of L-PL and D-PL were 20μg/m L and 5μg/m L respectively,the difference in differentiation rates of cells cultured on D-PL or L-PL at day 1 and2 couldn’t be eliminated,and the differentiation rate of cell cultured on D-PL was higher than that of L-PL.Among those 500,100 and20μg/m L of L-PL,100μg/ml L-PL had the highest differentiation rate.Similarly,among 125,25 and 5μg/m L of D-PL,although no statistical significance was found,25μg/m L D-PL showed a trend to have higher differentiation rate of nerve cells than other concentrations.Interestingly,bigger particle size was found in D-PL group than L-PL,it might contribute to the difference in the differentiation rates at early stage.Conclusion:L-PL and D-PL can affect the differentiation rate at the early stage of nerve cell growth,but do not affect the number of projections and the length of projections.