光动力疗法治疗金黄地鼠创伤性口腔溃疡的实验研究

Experimental Study of Photodynamic Therapy in the Hamsters Oral Ulcer Therapy

目的:探讨以亚甲基蓝为光敏剂的光动力疗法对金黄地鼠创伤性口腔溃疡的治疗效果。方法:金黄地鼠随机分成4组:空白对照组、光敏剂组、激光组、光动力组。取直径5 mm的金属棒,加热后置于金黄地鼠颊粘膜上5 s,肉眼可见形成直径约为5 mm的溃疡模型;按分组给予不同处理后,在6小时、24小时、5天、7天,处死制备病理切片,经HE、MASSON染色定性观察中性粒细胞数量、再生上皮、成纤维细胞、新生血管和胶原纤维情况。结果:模型建立24小时后,肉眼及组织病理学观察可见金黄地鼠口腔溃疡模型建立成功。在治疗24小时后,光动力治疗组的中性粒细胞数量与其他组相比显著增高(P<0.01)。治疗后第五天和第七天,新生的血管和胶原纤维情况光动力组明显优于空白对照组和光敏剂组(P<0.01),也多于激光组(P<0.05),上皮再生情况激光组优于空白对照组和光敏剂组(P<0.01),光动力组上皮再生也优于空白对照组和光敏剂组(P<0.05)。成纤维细胞数量各组间无明显差异。结论:光动力疗法可加快口腔溃疡的愈合。

Objective:To investigate oral ulcer-healing process after photodynamic therapy(PDT)mediated by methylene blue dye(MB).Methods:Golden hamsters were randomly divided into 4 groups:Control group,Ps group,LLLT group and PDT group.The lesions were made with a heated punch approximately 5 mm in diameter about 5 s and then given different intervention according the group.The animals in each group were killed at 6 hours,24 hours,5 days and 7 days and then sections were stained with hematoxylin and eosin(HE)and Masson trichrome(MT)for histological analysis by light microscopy in neutrophils,reepithelization stage,fibroblasts,new vessels and newly formed collagen.Results:After establishing oral ulcer model 24 hours,the HE staining showed that the model was successfully built.After 24 hours treating,in PDT group the neutrophils increased significantly compared with that in other groups(P<0.01).In 5 days and 7 days,the PDT group significantly improve the new vessels and newly formed collagen compared with that in Control group,Ps groups(P<0.01)and LLLT group(P<0.05).The LLLT group significantly improved the reepithelization stage compared with that in Control group and Ps groups(P<0.01)and the PDT group also improved the reepithelization stage compared with that in Control group and Ps groups(P<0.05).New fibroblasts did not show significant differences in four groups.Conclusions:PDT confers a protective effect against oral ulcer-healing response.