内切β-1,3-葡聚糖酶的分离纯化及性质研究

Purification and Properties of Endo-β-1,3-glucanase Produced from Schizophyllum Commune

对裂褶菌所产的内切β-1,3-葡聚糖酶粗酶液通过饱和硫酸铵沉淀,透析脱盐和Sephadex G-75柱层析进行纯化,纯化倍数为26.9倍,酶活回收率为33.8%。酶学性质研究表明,最适温度和pH分别为45℃和5.0,pH4.5-5.5范围内酶活相对稳定,热稳定性在60℃以下。Fe^(2+),Ba^(2+)对该酶有激活作用,Zn^(2+),K^+,Cu^(2+),Ag^(2+),Hg^(2+),Ca^(2+)对该酶有抑制作用。

The endo-β-1,3-glucanase from Schizophyllum commune Fr.was purified in three steps which comprised ammonium sulfate precipitation,DEAE- Sephadex A- 50 chromatography,and Sephadex G-100chromatography.The purified enzyme showed purification 14.60 fold,and an activity recovery of 11.6%.The optimal temperature and pH of the enzyme were 45℃ and 5.0 respectively.The β-1,3-glucanase was more stable at pH 4.5 ~5.5,and was relatively stable below 60℃.Zn^(2+),K^+,Cu^(2+),Ag^+,Hg^(2+),Ca^(2+)had inhibitory effect on the enzyme activity;Zn^(2+),Ca^(2+),Fe^(2+) and Ba^(2+) could stimulate the activity.