鼠精原干细胞体外培养影响因素的研究进展

The Researching Advances of Effective Factors on Spermatogonial Stem Cells of Muine Cultured in Vitro

精原干细胞(spermatogonial stem cells,SSCs)的体外培养是开展研究精原干细胞生物学的一项最基本的生物技术,近年来成为干细胞生物学研究的热点领域之一。如何获得高纯度、高活力的具有生物学安全性的SSCs是研究体外生物学的关键所在,而影响精原干细胞体外培养的因素很多,如动物种类和年龄、培养基、血清、饲养层的选择和生长因子等,长期以来一直认为精原干细胞无法在体外长期培养而且保持不分化,但随着研究的深入,尤其是鼠上,已经得到了SSCs体外长期培养。鼠作为研究哺乳动物甚至人常用的模拟生物,本文主要就近年来对鼠SSCs的研究现状及研究进展情况,并结合作者的工作经验,对体外长期培养鼠SSCs的影响因素作一详细评述。

In vitro culture of spermatogonial stem cells is a basic technology of biology,which can promote the biology investigation of spermatogonial stem cells.So spermatogonia! stem cells in vitro culture has become one of the hot fields in the Biology of the Stem Cell in recent years.Obtaining SSCs with high purity,plenty of vigor,biological safety are the essential steps for SSCs related biological studies in vitro.However,there are many factors affecting SSCs in vitro culture,such as animal species,age,culture medium,serum and selection of feeder layer and growth factors.SSCs always be thought not to culture in a long-term time in vitro,which retain stem cell activity.But with the deeper and deeper investigation,people have already obtained the long-time culture of SSCs,specially in Murine.Murine is a direct investigation analogue organism to research mammalian even human in the end.This review mainly summarize the factors,which affect SSCs in a long-term culture in vitro according to the recent related reports and our experimental work.