摘要
转基因技术的广泛应用对基因导入系统提出了越来越高的要求。构建能将目的基因高效稳定导入靶细胞的重组载体是制备转基因动物以及进行基因治疗的关键技术之一。基因导入系统可分为病毒载体与非病毒载体,病毒载体转染效率高、能介导外源基因稳定表达,因而在转基因技术应用中倍受关注。就病毒载体在转基因技术中的研究进展作一综述。
It is of primary importance to construct a suitable gene delivery system in the technology of transgenic animal generation and gene therapy,which can effectively mediate the exogenous genes expressed in the target cells.Vectors used for the genes transfer can be classified into virus vectors and non- virus vectors,thereinto,researches on virus vectors trend to be a hot spot in the technology of gene engineering attributing to its high efficiency of transfection and the stably expression of exogenous genes.However,because of the diversity of virus category,specificity of its structure and its complicated parasitic relationship with host organism,scientists are still working on being more familiar with its functional mechanism,life cycle and relationship with pathogenesis,which makes kinds of virus stiD not yet to be developed as gene delivery vector.Presendy,just a few kinds of virus have been successfully reconstructed as gene delivery system,and its progress in transgenic technology will be summarized in this essay.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第S1期230-234,共5页
China Biotechnology
基金
国家自然科学基金(30500270)
浙江省科技计划重点项目(2005C22052)
浙江省自然科学基金(Y304194)资助项目
