苯丙氨酸解氨酶(PAL)酶法合成L-苯丙氨酸的研究进展

Advance in the Production of L-phenylalanine by Using Phenylalanine Ammonia Lyase (PAL)

L-苯丙氨酸是人体必须但自身无法合成的八种必需氨基酸之一,是一种重要的医药和食用化学品中间体。到2005年,全球对L-苯丙氨酸的需求量达到3万吨,市场潜力巨大。特别是近年来,随着阿斯巴甜的全球热销以及底物反式肉桂酸生产成本的降低,利用苯丙氨酸解氨酶转化反式肉桂酸合成L-苯丙氨酸的生产工艺成为研究的热点。就苯丙氨酸解氨酶酶法合成L-苯丙氨酸的研究进展做了详细的阐述。

L-phenylalanine(L-Phe) is one of essential amino acids for humans.It is an important chemical intermediate in medicine and food industry.There is a huge market for L-Phe.The demand of L-Phe in all over the world reach 30000 ton in 2005.Especially,phenylalanine ammonia lyase(PAL) catalyzing trans-cinnamic acid(t-CA) to L-Phe method become hotspot in the production of L-Phe due to great demand for artificial sweetener aspartame and the descrease of the cost of tran-cinnamic acids.The progress in the production of L-phenylalanine by using PAL is summarized.