磁性纳米多孔复合支架材料的细胞相容性研究

Cytocompatibility research in magnetic nano-porous composite scaffold

目的研究磁性纳米多孔复合(n-HA/PLLA/Fe2O3)材料的细胞相容性,探讨细胞在材料表面黏附、增殖、表达等生物学行为,为其医学应用提供实验依据。方法将大鼠成骨细胞与磁性纳米多孔复合材料共培养,采用CCK-8法检测细胞增殖、扫描电镜观察细胞在材料上的黏附、RT-PCR检测I型胶原和骨钙素基因的表达。结果 CCK-8检测显示实验组磁性纳米多孔复合材料上细胞的增殖与空白对照组没有差异性(>0.05);扫描电镜观察到细胞在磁性纳米多孔复合材料的表面和孔隙内大量黏附、增殖和生长,随着共培养时间的增加,材料表面的细胞数量明显增多;RT-PCR显示随着共培养时间的增加,I型胶原基因的表达增强(<0.05),骨钙素的表达无明显差异(>0.05)。结论磁性纳米多孔复合支架材料适于成骨细胞的黏附、生长和分化,具有良好的细胞相容性。

Objective To study the magnetic nano-porous composite(n-HA/PLLA/Fe2O3) cell compatibility of materials to explore the cell surface adhesion, proliferation, expression and other biological behavior, and provide experimental evidence for its medical applications. Methods Rat osteoblasts and magnetic nano-porous composite co-cultured using CCK-8 cell proliferation assay, scanning electron microscopy of cell adhesion on the material, RT-PCR to detect the expression of type I collagen and osteocalcin genes. Results CCK-8 assay showed the experimental group on magnetic nanoporous composite cell proliferation and there was no difference between the control group(P>0.05); scanning electron microscope to a large number of cell adhesion and pores in the surface magnetic nano-porous composite material, proliferation and growth, with increasing time of co-culture, the number of cell surface was significantly increased; RT-PCR shows that with the increase of culture time, type I collagen gene expression enhanced(P<0.05), the expression of osteocalcin no significant difference(P>0.05). Conclusion Magnetic nano-porous composite scaffolds for osteoblast adhesion,growth and differentiation, has good biocompatibility.