摘要
CRISPR(Clustered Regularly Interspersed Short Palindromic Repeats)系统是一种细菌和古细菌用于对外源遗传物质进行降解的适应性免疫防御机制。其在基因组编辑方面的应用有着比其他核酸内切酶如锌指核酸酶(Zinc Finger Endonuclease,ZFNs)和类转录激活因子效应物核酸酶(Transcription Activator-like Effector Nuclease,TALENs)更好的功能。本文对该系统的分子机理、优势及仍然存在的问题进行阐述。随着对其研究的深入,该技术必将充分发挥其优势,在生命科学的发展中起到重要作用。
The CRISPR(Clustered Regularly Interspersed Short Palindromic Repeats)system comes from bacteria and archaea, which used in the degradation of foreign genetic material asadaptive immune defense mechanism. It is better than ZFN(Zinc Finger Nuclease) and TALENs(Transcription Activator-like Effector Nuclease) applied in the field of genomeediting. In this paper, we summarized the molecular mechanism, advantageand, disadvantage of the CRISPR system. We believe that with the development, this technology will play an important role in the modern life science.
出处
《生物化工》
2017年第2期73-79,共7页
Biological Chemical Engineering
