人乳头瘤病毒52型病毒样颗粒制备及其生物活性

Preparation and biological activity of human papillomavirus type 52 virus-like particles

[目的]利用大肠杆菌系统表达人乳头瘤病毒52型(HPV52) L1蛋白,并获得HPV52病毒样颗粒(VLPs)。[方法]优化并合成HPV52L1基因,构建p ET-30a-52L1表达载体粒,转化大肠杆菌E. coli BL21 StarTM(DE3)。经IPTG诱导表达,阳离子交换层析纯化,SDS-PAGE和Western Blotting鉴定,BALB/c小鼠免疫2次,初次后4w检测免疫血清中HPV52中和抗体滴度。[结果]获得纯度90%的HPV52L1蛋白,将获得的HPV52L1蛋白进行解组装和重组装形成VLPs,动态光散射观察到粒径约为70nm,透射电镜观察到50～60nm的均一VLPs,中和抗体滴度达25600。[结论]大肠杆菌系统表达人乳头瘤病毒52型(HPV52) L1蛋白,并经过一步柱层析纯化获得纯度达90%的L1蛋白,经解组装重组装获得粒径大小为50～60nm的VLPs,具有良好免疫原性。

[Objective]To express the major structural protein L1 of human papillomavirus type 52(HPV52)in E.coli and re-assemble L1 protein into virus-like particles(VLPs).[Method]HPV52 L1 gene was optimized and synthesized to construct p ET-30 a-52 L1 expression vector which was transformed into E.coli BL21 StarTM(DE3).The HPV52 L1 protein with purity of more than 90%was expressed that induced by IPTG,purified by cation exchange chromatography and identified by SDS-PAGE and Western Blotting.The HPV52 L1 protein was subjected to self-assembly VLPs with disassembly and reassembly.BALB/c mice were immunized for two times,HPV52 neutralizing antibody titer in immune serum was detected 4 weeks after the first immunization.[Result]Observation by dynamic light scattering showed that the uniform VLPs was formed in a round shape with a diameter about 70 nm and transmission electronic microscopy showed that about 50-60 nm.HPV52 neutralizing antibody titer in immune serum reached 25,600.[Conclusion]The human papillomavirus type 52(HPV52)L1 protein was expressed in E.coli system and purified by one-step column chromatography to obtain L1 protein with a purity of 90%.After disassembly and assembly,VLPs with particle size of 50-60 nm were obtained,has good immunogenicity.