软枣猕猴桃ICE1基因克隆与生物信息学分析

Cloning and bioinformatics analysis of ICE1 gene from Actinidia arguta Planch

[目的]为研究软枣猕猴桃ICE1基因的生物学功能及抗寒分子机制奠定基础。[方法]以4℃低温处理24 h的软枣猕猴桃(Actinidia arguta Planch) c DNA为模板,通过反转录PCR技术克隆ICE1基因。[结果]该基因c DNA长1524 bp,可编码507个氨基酸。生物信息学分析显示ICE1基因是编码MYC2型的b HLH转录因子,编码蛋白是定位于细胞核的非跨膜亲水蛋白。推导的氨基酸序列与中华猕猴桃(Actinidia chinensis var. chinensis)和山葡萄(Vitis amurensis) ICE1存在着较高的同源性。[结论]成功克隆到软枣猕猴桃抗寒转录因子命名为AaICE1。

Actinidia arguta Planch cDNA of 24 hours was treated at a low temperature of 4℃as a template,and ICE1 gene was cloned and named Aa ICE1.Its c DNA is 1 524 bp long and can encode 507 amino acids.Bioinformatics analysis shows that the ICE1 gene is a b HLH transcription factor encoding MYC2 type,and the coding protein is a non-transmembrane hydrophilic protein located in the nucleus.The derived amino acid sequences are homologous with Actinidia chinensis Planch and Vitis amurensis.The cloning of this gene laid the foundation for further research on the cold resistance mechanism of Actinidia arguta Planch.