产黄酮番木瓜内生真菌的筛选和鉴定

Isolation and Identification of Flavonoids-producing Endophytic Fungi in Papaya

为开发植物内生真菌这一微生物资源,采用组织分离法从番木瓜植株的叶片、果实和茎中分离内生真菌。通过黄酮类化合物显色反应、薄层层析法(TLC)、紫外光谱法(UV),对番木瓜内生真菌的次生代谢产物进行初步分析,筛选出1株产黄酮类物质的内生真菌菌株,并利用分生孢子形态特征和真菌内转录间隔区序列(Internal transcribed spacer,ITS)对该菌株进行分析鉴定。结果表明,从番木瓜果实中分离获得编号为G41的内生真菌菌株可归类为青霉属橘青霉(Penicillium citrinum)。研究证实番木瓜果实中存在可产黄酮类物质的内生真菌菌株,具有进一步开发利用的潜在价值。

In order to develop the microbial resources of endophytic fungi,endophytic fungi were isolated from the leaves,fruits and stems of papaya plants by tissue separation.The secondary metabolites of endophytic fungi from papaya were preliminarily analyzed by color reaction of flavonoids,thin layer chromatography,and UV spectroscopy.One strain of endophytic fungus producing flavonoids was screened,and the strain was analyzed and identified by using the conidial morphological characteristics and the internal transcribed spacer.The results showed that the endophytic fungus strain G41 isolated from papaya fruit could be classified as Penicillium citrinum.This study confirms that there are endophytic fungi strains producing flavonoids in papaya fruit,which demonstrates potential value for further development and utilization.