交联酶聚集体的制备及在漆酶固定化中的应用进展

Preparation of Cross-Linked Enzyme Aggregates and Its Application in Laccase Immobilization

漆酶是一种含铜的多酚氧化酶,其催化效率高,作用底物范围广,在工业中具有广阔应用前景。交联酶聚集体(CLEAs)是一种无需载体、无需酶纯化、制备简单、成本低的新型固定化酶方法,通常具有较高的稳定性,可实现酶的重复利用。对交联酶聚体制备过程中影响酶活性的相关因素以及其改进策略进行了分析,并对交联漆酶聚集体在污染物降解等方面的应用进行了总结,旨在为进一步的研究和应用奠定基础。

Laccase is a copper-containing polyphenol oxidase with high catalytic efficiency.Due to its broad substrate range,laccase has been widely used in many industrial fields.Cross-linked enzyme aggregates(CLEAs)technology is a novel carrier-free enzyme immobilization approach that could be simply performed using crude enzyme at low cost.The CLEAs usually have high stability and can be reused in multiple cycles.In this paper,we first analyzed the factors affecting enzyme activity during the CLEAs preparation and the related improving strategies,and then summarized the application of cross-linked laccase aggregates in pollutant degradation,with the aim of laying a foundation for further research and application of immobilized laccase.