摘要
脊髓灰质炎病毒的敏感细胞范围一直局限于灵长类动物细胞。用该病毒感染处理非灵长类的赤麂肺二倍体细胞(KIZ-7901),可诱导9.0—14.7%的细胞出现染色体畸变,除见有染色体或单体断裂外,还见有断片、双着丝粒、环、互换、易位和缺失等。也可引致姐妹染色单体交换率(SCE)的显著提高。脊髓灰质炎病毒感染赤麂细胞后,不产生特异的CPE,不明显影响细胞群体的增殖。应用病毒滴定、包涵体检查,间接免疫荧光试验和电镜检查,都可查见细胞内有病毒或其抗原物质存在。这提示脊髓灰质炎病毒在非灵长类的赤麂细胞内呈现非杀细胞性感染。
For a long time it has been reported that cells susceptible to poliovirusinfection only limited to primates.Weused the established Muntiacus mun-tjak lung diploid cell line (KIZ-7901)to study the biological effects of po-liovirus on the cells being infected withthem.The results showed that chro-mosome aberration rates of the cellsinfected with 100 TCD_(50) of the virusper cell were 9.0—14.7%,while thecontrol cells were only 3 %.Chromosome aberration types included chro-mosome and chromatid break,chromo-some fragment,dicentric chromosome,ring chromosome,interchange,trans-location,deletion and chromosomelysis.The broken sites mainly focused on the secondary constriction ofNO.1 chromosome and NO.3 chromo-some.It was found by the SCE analysis that all of the six virus strainsinduced significant higher SCE rates(11.04—13.62±0.48—0.64/cell) thanthat of the control (9.23±0.53/cell).No CPE was found after virus infection and no inhibition of cell divisionand proliferation as well.However,the infections virus particles appearedin the infected cells.The typicalacidophilic inclusion bodies and fluorescent positive cells including poliovirusantigen were found in some of theinfected cells.By using electromi-croscope examination,the virus-likeparticles with a diameter of about30nm scattering arround in plasmasmyelin figures and secondary lyso-somes were seen.It is thus clearlyindicated that poliovirus could produce a strong cell genetic effect andleads to nonkilling-cell infection inMuntiacus muntjak cells.
