诺如病毒GⅡ.13P蛋白的糖结合特征

Glycan binding pattern of norovirus GⅡ.13P protein

目的分析诺如病毒(norovirus,NoV)GⅡ.13毒株09N3145与组织血型抗原(histo-blood groups antigens,HBGAs)的结合特性。方法表达并纯化GⅡ.13 09N3145的P区蛋白,通过唾液及寡糖结合试验确定其与多种不同寡糖的结合特征,并以GⅡ.13/2010 P区蛋白与Lea寡糖复合物结构为模板,对GⅡ.13 09N3145 P蛋白进行同源模拟。结果 GⅡ.13 09N3145的P区蛋白可以与A、B、O+和非分泌型O-发生结合;可以与Lec双糖抗原和乳糖发生结合,而与A、B、H、Lewis型等寡糖无明显结合。分子模拟显示P蛋白使用一个可能的结合位点与半乳糖环结合。结论确定了GⅡ.13 09N3145 P蛋白与HBGAs的结合特征,为NoV宿主相互作用、人诺如病毒(human norovirus,huNoV)进化、流行以及天然抗病毒策略的提出奠定基础。

Objective To analyze the binding pattern between the P protein of GⅡ.13 strain(09 N3145)and histo-blood group antigens(HBGAs).Methods The P protein of GⅡ.13 09 N3145 was expressed and purified,of which the binding feature to various glycans was evaluated by saliva and glycan binding assays.A homology modeling of GⅡ.1309 N3145 P protein was built based on the complex structure of P protein of GⅡ.13/2010-Lea.Results GⅡ.1309 N3145 P protein showed binding to the types A,B and O+secretor as well as O-non-secretor saliva.However,the P protein showed binding to Lec disaccharide antigens and lactose,while no significant binding to oligosaccharides of types A,B,H and Lewis.The homology modeling indicated that P protein bound to galactose ring using a potential glycan binding site.Conclusion The binding pattern between P protein of GⅡ.13 09 N3145 and HBGAs was determi-ned.These findings provided a basis for elucidating the mechanism of evolution,epidemiology and natural antiviral strategy of human norovirus(huNoV).