细菌基因敲除策略及其在维氏气单胞菌研究中的应用

Application of gene knockout techniques of bacteria in study on Aeromonas veronii

基因敲除技术是20世纪80年代发展起来的一种分子生物学技术,目前已广泛应用于微生物基因功能等相关领域的研究中。维氏气单胞菌(Aeromonas veronii,A. veronii)是气单胞菌科气单胞菌属的一种,近年来,该菌引发疾病的报道逐年增多,因此,对其致病机制的研究显得尤为重要。本文对细菌不同基因敲除策略及其在A. veronii基因功能研究中的应用作一综述,旨在为A. veronii致病机制的深入研究提供参考。

Gene knockout is a molecular biological technique developed in 1980 s,which has been widely used for the studies in relevant fields such as gene function of microorganism.The diseases caused by Aeromonas veronii increased year by year,thus it is important to study the pathogenic mechanism.This paper reviews the application of various gene knockout strategies in the study on gene function of A.veronii in order to provide a reference for further study on pathogenic mechanism of the bacterium.