毛细管柱内在线反应化学发光测定抗坏血酸

Determination of ascorbic acid based on the combination of capillary electrophoresis and chemiluminescence

建立了一种毛细管柱内反应毛细管区带电泳分离化学发光检测痕量抗坏血酸的新方法。方法基于酸性重铬酸钾在毛细管柱内与抗坏血酸发生氧化还原反应生成具有化学发光催化活性的Cr(Ⅲ),通过毛细管电泳分离化学发光法检测Cr(Ⅲ)间接地测定抗坏血酸的含量。电泳缓冲液由0.02mol/L HAc-NaAc(pH 4.7)和1×10-3 mol/L EDTA溶液组成。对影响分离和检测的各参数都进行了优化实验。抗坏血酸检测限(3σ)为6×10-11 mol/L。该方法具有简单、灵敏度高、选择性好等特点,可用于血液中痕量抗坏血酸的测定。

A sensitive method for the determination of ascorbic acid based on the combination of electrophoresis separation and chemiluminescence was developed.The method was based on the reduction of Cr(Ⅵ)to Cr(Ⅲ)with ascorbic acid occurring inside the capillary and the light emission produced by luminol oxidation by hydrogen peroxide in basic aqueous solution catalyzed by Cr(Ⅲ).Running buffer was composed of 0.02mol/L HAc-NaAc(pH 4.7)with 1×10-3 mol/L EDTA,and parameters affecting CE-CL separation and detection were optimized.The limit of detection for ascorbic acid(3σ)was 6×10-11 mol/L.This method offered potential advantages of simplicity,sensitivity,selectivity and applicability to the determination of ascorbic acid in blood samples.