PEP-3与HBc融合原核表达载体的构建与表达

The construction and expression of the prokaryotic expression vector of HBc-VLPs displaying PEP-3 peptide

以可诱导较强免疫反应及呈现高拷贝抗原小肽的乙肝病毒核心蛋白(Hepatitis B Virus Core Protein,HBc)类病毒颗粒(Virus-like Particles,VLPs)作为免疫载体蛋白,通过分子生物学手段,将蓝白班筛选元件Lac Zα插入HBc的主要免疫显性区域,构建获得外源小肽与HBc融合的通用原核表达载体。在此通用载体的基础上,通过PEP-3小肽替换Lac Zα片段,构建了PEP-3与HBc融合原核表达载体,并成功进行了HBc/PEP-3融合蛋白的表达及纯化。该新型通用载体的成功构建,将为小肽疫苗的研究及应用提供有效工具。

Hepatitis B virus core protein like particles(HBc-VLPs),which have a strong immune response and present the high copy number of antigen epitope,were used as epitope carriers.By taking methods of molecular biology,a white/blue screening element Lac Zαwas placed into the major immunodominant region of HBc-VLPs,and the prokaryotic expression vector for HBc con-taining the epitope peptide was constructed.Based on the vector,the prokaryotic expression vec-tor of HBc/PEP-3 was constructed by replacing Lac Zαelement with PEP-3 peptide consisting of 1 3 amino acids,then the expression and purification of fusion protein HBc/PEP-3 were success-fully carried out.The construction of the new universal vector will provide an efficient tool for the research and application of peptide vaccine.