沉默AFAP-1L2对胃癌MKN-28细胞的生物学影响

The biological effects on gastric MKN-28 cells by silencing AFAP-1L2

目的:探讨AFAP-1L2对胃癌MKN-28细胞的生物学影响。方法:siRNA干扰AFAP-1L2;CCK-8检测沉默AFAP-1L2后细胞的增殖情况;免疫荧光分析各组细胞的凋亡率;Transwell小室观察细胞的侵袭情况;Real time-PCR检测细胞中基因的表达情况。结果:siRNA有效干扰了AFAP-1L2的表达;沉默AFAP-1L2细胞组的细胞增殖明显受到抑制(P<0.05);siRNA-AFAP-1L2组细胞的凋亡率与其他组比较,凋亡率增高;细胞的侵袭情况,沉默AFAP-1L2明显抑制了细胞的侵袭,与其他两组比较差异显著(P<0.05);ERK1/2、p-ERK1/2和Bcl-2的表达水平,均是siRNA-AFAP-1L2细胞组低于其他两组(P<0.05)。结论:沉默AFAP-1L2抑制了胃癌MKN-28细胞的增殖,诱导细胞的凋亡,抑制了细胞的侵袭和抗凋亡基因的表达,AFAP-1L2可以通过ERK1/2途径发挥作用,为肿瘤的靶向治疗提供一定的理论依据。

Objective: To explore the biological effects on gastric MKN- 28 cells by AFAP- 1L2. Methods: siRNA interfer AFAP- 1L2. Using CCK- 8 to detect the proliferation of cells with AFAP- 1L2 silence. To analyse the condition of cell apoptosis by immunofluroescence. Using Transwell to detect the invasion ability. The expression of genes in MKN- 28 cells were analysed by Real time- PCR. Results: siRNA effectively interfered the expression of AFAP- 1L2. Cell proliferation was significantly suppressed in siRNA- AFAP- 1L2 cell group( P < 0. 05). The apoptosis rate of siRNA- AFAP- 1L2 was significantly higher than other groups. The cell counts of siRNA- AFAP- 1L2 group was lower than other groups( P < 0. 05). Real time- PCR showed that the expression of ERK1 /2,p- ERK1 /2and Bcl- 2 in siRNA- AFAP- 1L2 group was lower than those in other groups( P < 0. 05). Conclusion: Silence of AFAP- 1L2 inhibited gastric cancer MKN- 28 cell proliferation and induced the apoptosis of cells,inhibited invasion and antiapoptotic gene expression,AFAP- 1L2 may play a role by ERK1 /2 signaling pathway,these results provided certain theoretical basis for targeting therapy of gastric cancer.