LPS诱导宫颈癌上皮间质转化的作用

LPS induced epithelial-mesenchymal transition of human cervical cancer

目的:探讨LPS在宫颈癌细胞株Hela细胞增殖和上皮间质转化中的作用。方法:体外培养Hela细胞,以LPS(10μg/ml)诱导后,利用MTT法检测细胞的增殖情况。q PCR检测上皮细胞标志物E-cadherin和间质细胞标志物N-cadherin、Vimentin的RNA水平上的表达。Western blot检测上皮细胞标志物E-cadherin和间质细胞标志物N-cadherin、Vimentin的蛋白水平上的表达。Transwell检测LPS对Hela细胞迁移能力的影响。结果:在LPS诱导后能够明显的促进Hela细胞的增殖。E-cadherin在RNA和蛋白水平上都明显下降,N-cadherin、Vimentin在RNA和蛋白水平上都明显升高;LPS诱导后,明显促进Hela细胞的迁移能力。结论:LPS能够诱导宫颈癌细胞株Hela细胞的增殖和上皮间质转化。

Objective: To investigate the effect of LPS on proliferation and epithelial- mesenchymal transition( EMT) of human cervical cancer. Methods: A549 cells cultured in vitro were treated by LPS( 10μg/ml). The effect of LPS on proliferation was detected by MTT. The RNA level effect of LPS on epithelial markers( E- cadherin) and mesenchymal markers( N- cadherin,Vimentin) was detected by q PCR. The protein level of LPS on epithelial markers( E- cadherin) and mesenchymal markers( N- cadherin,Vimentin) was detected by Western blot. The effect of LPS on migration of Hela cell was detected by Transwell assay. Results: After treated by LPS,significantly promote the proliferation of Hela cell. The RNA and protein level of epithelial markers( E- cadherin) was decreased,and mesenchymal markers( N- cadherin,Vimentin) was increased. Transwell test showed that the migration potential of Hela cells was significantly increased after LPS treatment. Conclusion: LPS can induce cervical cancer cell line Hela proliferation and epithelial- mesenchymal transition