PKM2在食管鳞癌组织中的表达及其临床意义

The expression of PKM2 in human esophageal squamous cancer cell and its clinical significance

目的:探讨食管癌中丙酮酸激酶2(PKM2)的表达情况及临床意义。方法:收集川北医学院附属医院外科手术切除并经病理确诊的食管鳞癌30例(其中高分化9例、中分化10例、低分化11例)及正常组织(包含切缘组织)标本30例,采用免疫组化方法检测PKM2的表达。结果:PKM2在正常食管组织、切缘组织、食管鳞癌组织中的阳性表达率分别为0、0、90%,PKM2在食管鳞癌组织中的表达与正常组织、切缘组织的表达差异有统计学意义(P<0.001)。在食管癌鳞癌中,不同浸润程度的食管鳞癌组织PKM2的表达差异有统计学意义。PKM2的表达水平在不同分化程度、是否淋巴结转移中的差异无显著性意义。结论:PKM2在食管鳞癌组织中存在显著高表达。

Objective: To study the M2 type of pyruvate kinase( PKM2) 's expression in human esophageal squamous cell carcinoma and its clinical significance. Methods: Specimens were collected,included 30 cases of esophageal squamous cell carcinoma and 30 cases margin tissue. Specimens were stained through IHC with PKM2 antibodies. Results: The rates of PKM2 positive expression in margin tissues and esophageal squamous cell carcinoma were 0 and90% respectively. There was significant difference of PKM2 expression between esophageal squamous cell carcinoma tissues and margin tissues( P < 0. 001). In esophageal squamous cell carcinoma,PKM2 expression difference was statistically significant in infiltrating various levels of ESCC. The positive expression of PKM2 was not significantly correlated with degree of differentiation and lymph node metastasis. Conclusion: PKM2 is highly expressed in human esophageal squamous cell carcinoma tissues.