miRNA-145在胃癌中的表达及临床意义

Expression of miRNA-145 in gastric cancer and its clinical significance

目的:探讨miRNA-145在胃癌组织中的表达及其与临床病理特征、预后之间的关系。方法:应用实时定量RT-PCR(qRT-PCR)检测miRNA-145在63例胃癌组织和40例对照组织中的表达,并将miRNA-145的含量分为高表达组和低表达组,分析其与胃癌临床病理特征、预后的关系。结果:miRNA-145在胃癌组织中的表达低于对照组,具有显著性差异(P<0.000 1)。miRNA-145的表达与患者的性别、年龄以及肿瘤的大小无关(P>0.05)。与分化程度、TNM分期、远处转移及术后3年生存率有关(P<0.05)。结论:miRNA-145的表达与胃癌的发生、发展及预后有关,miRNA-145有望成为胃癌新的肿瘤标志物之一。

Objective: To study the miRNA- 145 expression in gastric cancer tissue and its relationship with the clinical pathologic features and prognosis. Methods: qRT- PCR was applied to detect the expression of miRNA- 145 in 63 cases of gastric cancer and 40 cases of control group. The contents of miRNA- 145 were divided into high expression and low expression group,and the relationship between miRNA- 145 contents and the clinical pathological features and prognosis was analyzed. Results: The level of miRNA- 145 in gastric cancer group was lower than that in the control group tissues,which had a significant difference( P < 0. 000 1). miRNA- 145 expression in the gastric cancer was not correlated with the patient's age,gender and tumor size( P > 0. 05). There were associations with TNM stage,degree of differentiation,distant metastasis and 3- year survival rate( P < 0. 05). Conclusion: Expression of miRNA- 145 is correlated with the occurrence,development and prognosis of gastric cancer. miRNA- 145 is likely to be one of new tumor markers for gastric cancer.