肺癌缺氧A549细胞的microRNA芯片表达谱分析

MicroRNA expression profiling analysis of hypoxic cells in human lung adenocarcinoma

目的:研究缺氧对肺腺癌A549细胞中microRNA表达谱的影响,分析靶基因参与的生物学功能和通路。方法:基于miRNA芯片技术和生物信息学分析方法,分析缺氧条件和正常氧条件下培养的A549细胞中差异表达的miRNA,并预测这些miRNA的靶基因,分析靶基因参与的生物学功能和通路。结果:本研究共筛选出14个差异表达miRNA,包括9个在缺氧细胞中上调miRNA和5个下调miRNA。上调和下调miRNA的靶基因都参与DNA转录、核染色质修饰等功能,并且都参与Wnt信号、TGF-β信号和MAPK信号通路。结论:缺氧的肺腺癌A549细胞中miRNA表达谱发生了显著改变,差异表达miRNA对某些基因具有调控作用。

Objective: To analyze the effect of hypoxia on miRNA expression in lung adenocarcinoma A549 cells.Methods: Based on miRNA chip technology and bioinformatics analysis methods,differentially expressed miRNAs between hypoxia- treated A549 cells and normoxia- treated cells were identified,and target genes of them were predicted. Besides,biological functions and pathways of targets were analyzed. Results: In total,14 differentially expressed miRNAs were screened,including 9 upregulated miRNAs and 5 downregulated miRNAs in hypoxia- treated A549 cells. Target genes of both upregulated and downregulated miRNAs were enriched in GO terms,such as DNA transcription,chromatin modification,as well as a set of pathways,such as Wnt signaling,TGF- beta signaling and MAPK signaling. Conclusion: In the hypoxic A549 cells,miRNA expression was significantly changed. A series of differentially expressed miRNAs regulated certain genes.